Fluorescence spectroscopy measures light emitted by a sample after it absorbs photons at a specific excitation wavelength. The emitted light is always at a longer wavelength (lower energy) than the excitation light — a shift known as the Stokes shift. This technique is extraordinarily sensitive, capable of detecting fluorophores at nanomolar concentrations, and provides both excitation and emission spectra that serve as unique identifiers.
Fluorescent protein spectra, dye emission profiles, and other fluorophore reference data underpin applications in molecular biology, medical diagnostics, environmental sensing, and materials characterization. Emission spectra are essential for designing multi-color imaging experiments, selecting filter sets, and deconvolving overlapping signals in multiplexed assays. Accurate reference spectra prevent spectral crosstalk and optimize signal-to-noise ratios.
SpectralBench hosts fluorescence spectroscopy reference spectra with both excitation and emission data. Browse interactive spectra, compare fluorophore profiles, and download in CSV or JCAMP-DX for integration with your microscopy, flow cytometry, or plate-reader workflows.